The endemic Australian bee genus Trichocolletes is revised. Forty species are recognised, including twenty-three new species: Trichocolletes aeratus, T. albigenae, T. avialis, T. brachytomus, T. brunilabrum, T. capillosus, T. centralis, T. dundasensis, T. fuscus, T. gelasinus, T. grandis, T. lacaris, T. leucogenys, T. luteorufus, T. macrognathus, T. micans, T. nitens, T. orientalis, T. platyprosopis, T. serotinus, T. simus, T. soror and T. tuberatus. Four new synonymies are proposed: Paracolletes marginatus lucidus Cockerell, 1929 = T. chrysostomus (Cockerell, 1929); T. daviesiae Rayment, 1931 = T. venustus (Smith, 1862); T. marginatulus Michener, 1965 = T. sericeus (Smith, 1862); T. nigroclypeatus Rayment, 1929 = T. venustus (Smith, 1862). Trichocolletes rufus (Rayment, 1930) is moved to Leioproctus and T. rufopilosus (Rayment, 1935) to Anthoglossa. Descriptions are given for new species and redescriptions for species described before 1965, including first descriptions of males of T. aureotinctus (Cockerell), T. burnsi Michener, T. latifrons (Cockerell) and T. maximus (Cockerell) and the females of T. dowerinensis Rayment and T. rufibasis (Cockerell). Lectotypes are designated for Lamprocolletes venustus Smith and Anthoglossa plumata Smith. Keys to species are provided for both sexes, as are distribution maps and a summary of recorded floral visitation.